Day 1

What did you do today?
Today we collected bacteria samples from around the school, and prepared them by Swabbing on the agar-filled petri dishes together with a few more provided bacteria to cultivate, so that they can be tested on tomorrow.

Swabbing:


What did you learn today?

-Bacteria can be BOTH Helpful and Harmful
-They can be classified into 3 shapes
1. Sphere(Coccus)
2. Spiral(Spirillum)
3. Rod(Bacillus)

-Some kinds of bacteria produce Oxygen (Approx. 1/2 the oxygen we breathe is produced by bacteria)
Fun Fact: There is approx. 2kg of bacteria in our body
-Learnt how to sterilise equipment before using them (Test tubes, working spaces, etc)(Using the Methylated Spirit Lamp)

-Lab Safety(Wearing gloves, be careful of flammable substances, etc)
-How to properly spread the bacteria on the inoculating loop on the agar (Swabbing)
-Definition of Pathogenic Bacteria: Disease-causing bacteria

How do you feel about today’s activities? 

I felt that today's activities were very interesting and they gave me an insight on what it is like to work in a laboratory.

Other Questions:

1. What are Bacteria? List down some characteristics of bacteria
Bacteria are microscopic organisms that can be BOTH Harmful and Helpful
Bacteria are unicellular and prokaryotic( Nucleus Absent)

2. How can bacteria be identified

2 Ways to identify the Genus of Bacteria found outside the body:
Gram Staining 
-Bacteria are stained with dye
-They are then washed with Potassium Iodide
-Bacteria that RETAIN the dye after washing are known as Gram-Positive Bacteria
-Bacteria that LOSE the dye after washing are known as Gram-Negative Bacteria




Ziehl-Neelsen Staining.
-Used to identify bacteria that are unable to be stained due to characteristics like Waxy Coats on their cell walls
-Detergents are used to remove the waxy coating before the dye is used

3. Name 3 common bacteria and places they can be found
E. coli, Vibrio Cholerae and salmonella
Kitchen Sinks and Uncooked Food.

4. Are ALL bacteria harmful? If not, name some bacteria that are beneficial to humans
No, not all bacteria are harmful.
Types of beneficial bacteria( aka Probiotics )
-Lactobacillus acidophilus
-Lactobacillus casei
^Both of the above Probiotics can be found in Vitagen


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